![]() This may be repeated multiple times (booster doses of antigen). The immunization of animals is achieved by injecting the antigen with a suitable adjuvant (non-antigenic preparation known to stimulate an immune response). This animal is previously immunized with the antigen against the desired monoclonal antibodies. The isolation of B-lymphocytes takes place from the spleen of an animal like a Mouse. The steps to produce the monoclonal antibodies are as follows The fusing agent can be a defective virus (Sendai virus that characteristically causes cell fusion) or chemicals like polyethylene glycol. And this hybridoma possesses the immortal growth properties of the myeloma cell and the antibody-secreting property of the B-cell.The hybrid cell, which is produced as the result of this fusion, is called a hybridoma.In this method, the monoclonal antibodies are produced by fusing usually activated antibody-producing B-cells with myeloma cells.In the Vivo method (Using living animals like Mice) In Vitro Method.In Vitro Methods (Using cell lines in glassware).Monoclonal antibody production can be done by two methods. This quickly became one of the immunology’s key technologies, and they were awarded the Nobel Prize for the same in medicine and physiology in 1984. In 1975, two scientists, Georges Kohler and Cesar Milstein, devised a revolutionary method for preparing monoclonal antibodies. All molecules of a monoclonal antibody will have the same specificity. Therefore, such antibodies are known as monoclonal antibodies. This clone produces antibodies of a single specificity. The clone derived from the fusion of a single differentiated B-cells(antibody-forming) is a clone of a single B-cell. ![]() These hybrid cells express the properties of both parental cell lines. ![]()
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